000 02306nam a22003137a 4500
003 OSt
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022 _a0021-9266
040 _cDSSC
082 _aPer/668/N859/2016
100 _aNovo, M. Teresa
_911582
130 _aJournal of Biological Education
_911209
245 _aHow do detergents work: A qualitative assay to measure amylase activity
_cby M. Teresa Novo, Marina Casanoves, Santi Garcia-Vallve, Gerard Pujadas, Miquel Mulero and Cristina Valls
300 _a251-260
336 _atext
337 _aunmediated
338 _avolume
440 _v50(3)
_x0021-9266
500 _aWe present a practical activity focusing on two main goals: to give learners the opportunity to experience how the scientific method works and to increase their knowledge about enzymes in everyday situations. The exercise consists of determining the amylase activity of commercial detergents. The methodology is based on a qualitative assay using a colorimetric process. Quantitative results are also obtained by measuring the halo formed. This activity is suitable for and adaptable to learners at different levels of education: primary school, secondary education or even for pre-service teachers, which is the group the version described in this paper was intended for. This laboratory activity was designed to include the scientific method as a learning outcome. This was especially important in pre-service teachers, as increasing scientific literacy is one of the primary goals of science education. Through the activity, students also learn about micro-organisms and their applications in their daily lives, which is one of the tenets of Science-Technology-Society-Environment programs. A case study was conducted with a group of learners made up of 75 pre-service teachers from Universitat Rovira i Virgili in Tarragona, in order to verify whether this lab activity is well designed and can be satisfactorily implemented.
650 0 _aDetergent
_911583
650 0 _aMolecular biology
_94708
700 _aCasanoves, Marina
_911584
700 _aValls, Cristina
_911588
700 _aMulero, Miguel
_911587
700 _aPujadas, Gerard
_911586
700 _aGarcia-Vallve, Santi
_911585
942 _2ddc
_cPINDEX
999 _c8315
_d8315