02844nam a22002897a 4500 OSt 20261014132317.0 261014b |||||||| |||| 00| 0 eng d 1738-1266 DSSC Jeong, Jin-Tae 13710 Journal of Biosystems Engineering 13657 A dielectrophoresis microfluidic device for trapping bioparticles at low voltage and frequency / Jin-Tae Jeong, Hyun-Min Shin, Duwoon Kim, and Kyeong-Hwan Lee Korea : The Korean Society for Agricultural Machinery, 2016. pp. 60-65 text unmediated volume 41(1), 60-65 1738-1266 Purpose: The necessity for precise manipulation of bioparticles has greatly increased in the fields of bioscience, biomedical, and environmental monitoring. Dielectrophoresis (DEP) is considered to be an ideal technique to manipulate bioparticles. The objective of this study is to develop a DEP microfluidic device that can trap fluorescent beads, which mimic bioparticles, at the low voltage and frequency of the sinusoidal signal supplied to the microfluidic device. Methods: A DEP microfluidic device, which is composed of polydimethylsiloxane (PDMS) channels and interdigitated electrode networks, is fabricated to trap fluorescent beads. The geometry of the interdigitated electrodes is determined through computational simulation. To determine the optimum voltage and frequency of the sinusoidal signal supplied to the device, the experiments of trapping beads are conducted at various combinations of voltage and frequency. The performance of the DEP microfluidic device is evaluated by investigating the correlation between fluorescent intensities and bead concentrations. Results: the optimum ratio of the widths between the negative and positive electrodes was 1:4 (20:80 um) at a gap of 20 um between the two electrodes. The DEP electrode networks were fabricated based on this geometry and used for the bead trapping experiments. The optimum voltage and frequency of the supplied signal for trapping fluorescent beads were 15 V and 5 kHz, respectively. The fluorescent intensity of the trapped beads increased linearly as the bead concentration increased. The coefficient of determination (R2) between the fluorescent intensity and the bead concentration was 0.989. Conclusion: It is concluded that the microfluidic device developed in this study is promising for trapping bioparticles, such as a cell or virus, if they are conjugated to beads, and their concentration is quantified. Bead manipulation 13711 Bioparticle 13712 Dielectrophoresis 13713 Fluorescent intensity 13714 Microfluidic device 13715 ddc PINDEX 10431 10431 0 0 ddc 0 0 Per SPMT-CAS SPMT-CAS PER 2026-10-14 0 2026-10-14 13:23:27 2026-10-14 PINDEX